Characterization of NarJ, a System-specific Chaperone Required for Nitrate Reductase Biogenesis in Escherichia coli
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چکیده
منابع مشابه
Structural and biochemical characterization of a quinol binding site of Escherichia coli Nitrate Reductase A (NarGHI)
◊ Department of Biochemistry, University of British Columbia, 2146 Health Science Mall, Vancouver, British Columbia, V6T 1Z3 ‡ CIHR Membrane Protein Research Group, Department of Biochemistry, 474 Medical Sciences Building, University of Alberta, Edmonton, Alberta T6G 2H7. ¶ Laboratoire de Chimie Bactérienne, CNRS, 31 chemin Joseph Aiguier, 13402 Marseille Cedex 9, France + UMR6191, LBC, CEA Ca...
متن کاملStructural and biochemical characterization of a quinol binding site of Escherichia coli nitrate reductase A.
The crystal structure of Escherichia coli nitrate reductase A (NarGHI) in complex with pentachlorophenol has been determined to 2.0 A of resolution. We have shown that pentachlorophenol is a potent inhibitor of quinol:nitrate oxidoreductase activity and that it also perturbs the EPR spectrum of one of the hemes located in the membrane anchoring subunit (NarI). This new structural information to...
متن کاملImmunochemical localization of nitrate reductase in Escherichia coli [proceedings].
The membrane-bound nitrate reductase from Escherichiu coli functions as the terminal enzyme of the respiratory chain of the organism when it is grown anaerobically in the presence of nitrate. The enzyme consists of two types of polypeptide chain, a and 8, of approx. mol.wts. 155000 and 65000 respectively (Enoch &Lester, 1975). In conjunction with cytochrome bN%6it constitutes a proton-transloca...
متن کاملChaperone properties of Escherichia coli thioredoxin and thioredoxin reductase.
Thioredoxin, thioredoxin reductase and NADPH form the thioredoxin system and are the major cellular protein disulphide reductase. We report here that Escherichia coli thioredoxin and thioredoxin reductase interact with unfolded and denatured proteins, in a manner similar to that of molecular chaperones that are involved in protein folding and protein renaturation after stress. Thioredoxin and/o...
متن کاملNitrate reductase and related enzymes in Escherichia coli.
Several reports about nitrate reduction in Escherichia coli have appeared during the last few years. Taniguchi, Sato & Egami (1956) have found in cell-free extracts of E. coli, grown in a peptone broth-agar medium containing nitrate, a particulate system which reduces nitrate to nitrite, only under anaerobic conditions and with reduced diphosphopyridine nucleotide, formate or reduced methylene ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1997
ISSN: 0021-9258
DOI: 10.1074/jbc.272.39.24266